gram negative bacteria in gelatin hydrolysis test

Different microorganisms could be applied for Petroleum hydrocarbons biodegradation. 50–98% of crude oil is Petroleum hydrocarbons which considered a major component depending on the source of the oil. Petroleum (crude) oil are compounded of thousands compounds mixture. Remarkable degradation rate % patterns for hydrocarbons residual were recorded for all three isolates with varied. Different features were studied for three biodegrading bacterial strains and identified as Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereus. Biodegradation patterns for different three isolates were reflected varied degradation ability for three isolates regarding incubation time. Phyllogenetic tree was constructed and genetic similarity was calculated according to alignments results. Interestingly, 16S rDNA sequencing fingerprinting results confirmed our bacterial identification as Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereu. Three bacterial strains were selected from petroleum-contaminated soils of Khurais oil field depending on their capacity to grow in the existence of hydrocarbon components and identified according to morphological, biochemical. Furthermore, degradation level in contaminated liquid medium and soil were evaluated. The aim of this study is isolation and identification biodegradable capability bacteria from contaminated sites in Khurais oil field, Dhahran, Saud Arabia via Different morphological and biochemical and molecular methods. Until now, In situ biodegradation considered the most effective method for petroleum hydrocarbon residual biodegradation. Accumulation of petroleum hydrocarbon residual considered a major environmental problem in the kingdom of Saudi Arabia cause of intensive efforts for oil detecting.

Gram negative bacteria in gelatin hydrolysis test